Survival of bone marrow mesenchymal stem cells after transplantation into the rat infarcted myocardium

doi:10.3969/j.issn.2095-4344.2014.41.012

Abstract

Abstract:

BACKGROUND: The preliminary findings confirm that bone marrow mesenchymal stem cell transplantation is safe and effective in the treatment of acute myocardial infarction, but its exact mechanism is unclear. There are few studies addressing the survival status of transplanted stem cells and its acting timing.

OBJECTIVE: To study the survival of rat bone marrow mesenchymal stem cells transplanted into the infracted myocardium.

METHODS: Bone marrow mesenchymal stem cells were cultured using density gradient centrifugation. Eighty rat models of myocardial infarction were prepared. Bone marrow mesenchymal stem cells were injected via a microsyringe at four sites around the infarcted region at 14 days after modeling. Then, 70 rats with living cells were selected for detecting the survival of bone marrow mesenchymal stem cells at days 3, 5, 7, 10, 20, 28 after transplantation.

RESULTS AND CONCLUSION: Under ×400 visual field, the number of Brdu-positive bone marrow mesenchymal stem cells was (36±12) at 3 days posttranplantation, (33±13) at 5 days, (28±9) at 7 days, (15±5) at 10 days, (5±3) at 14 days, 0 at 20 days, and 0 at 28 days, showing a overall downward trend after transplantation. The number of bone marrow mesenchymal stem cells was negatively correlated with transplant days (P < 0.01, r=-0.47). The number of cells decreased most significantly within 1 week after transplantation, and then decreased to 0 at 20 days. These findings indicate that transplanted bone marrow mesenchymal stem cells in the myocardium cannot survive for a long term and also cannot be transformed into myocardial tissue.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, myocardial infarction, models, animal

CLC Number:

R394.2

Lin Chu-wei, Zhou Sheng-hua, Dai Hai-ying, Deng Ping, Huang Hong-guang, Yin Zhi-lan, Guan Xian-song. Survival of bone marrow mesenchymal stem cells after transplantation into the rat infarcted myocardium[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6628-6632.

Figures/Tables 1

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